Workpackage 1: Metabolic engineering of light-driven in vivo biotransformations
WP1 compares different types of transformations in cyanobacteria and explores tools for their optimization. The enzymes are cloned in shuttle vectors under the control of constitutive and inducible promoters. After verification of the functional expression, activity and substrate spectrum are investigated and the reaction conditions optimized. The enzymes will be linked to Synechocystis mutants with optimized electron flow.
Workpackage Leader: Prof. Dr. Paula Tamagnini
Lead beneficiary: University of Porto