Engineering ferredoxin-dependent oxyfunctionalization in cyanobacteria

Fellow: ESR3
Home Institution: University of Porto – Portugal
Academic Secondment: Aix Marseille University – France
Industrial Secondment: Innophore GmbH – Austria


This research will focus on the introduction of limonene hydroxylase in cyanobacteria; implementation of a ferredoxin-dependent monooxygenation (synthesis of perrillyl alcohol from limonene by P450 monooxygenase); targeted identification of novel monooxygenases for light-catalyzed whole-cell monooxygenation (Computer aided search tools based on 3D-structure and sequences); and fusion protein coupling ferredoxin and P450 and comparison.